標準號：DIN ISO 16000-17-2010
中文標準名稱：室內空氣.第17部分:霉菌的測定與計算.培養基方法(ISO 16000-17-2008)
英文標準名稱：Indoor air - Part 17: Detection and enumeration of moulds - Culture-based method (ISO 16000-17:2008)
標準類型：C51
發布日期：2010/6/1 12:00:00
實施日期：1999/12/31 12:00:00
中國標準分類號：C51
國際標準分類號：13.040.20
引用標準：ISO 8199;ISO 16000-16;ISO 16000-18
適用范圍：Mould spores are widely distributed in the outdoor environment and, therefore, occur in varying concentrations also indoors. Growth of moulds in indoor environments, however, should be considered a hygienic problem because epidemiological studies have revealed that dampness and/or mould growth in homes and health problems affecting the occupants are closely related. Harmonized methods for sampling, detection and enumeration of moulds including guidelines for sampling strategies are important for comparative assessment of mould problems indoors. Sampling is carried out according to the method described in DIN ISO 16000-16 or -18. Agar plates (DG 18 agar and malt-extract agar or potato extrose agar) obtained from sampling by impaction are incubated directly at (25 +/- 3) ?C and (36 +/- 2) ?C. Filters obtained from sampling by filtration are re-suspended in saline solution (0, 9 % NaCl) with 0, 01 % Polysorbate 80. Decimal dilutions of the suspension are prepared and aliquots spread on DG 18 agar as well as on malt-extract agar or potato dextrose agar (indirect method). Agar plates are incubated at (25 +/- 3) ?C. For special purposes plates can be incubated at (36 +/- 2) ?C (e. g. thermotolerant Aspergillus spp.) or (45 +/- 2) ?C (Aspergillus fumigatus). After incubation, mould colonies are identified and counted.