標準號：DIN 55672-3-2007
中文標準名稱：凝膠滲透色譜法(GPC).第3部分:用水作洗脫溶劑
英文標準名稱：Gel permeation chromatography (GPC) - Part 3: Water as elution solvent
標準類型：G04
發布日期：1999/12/31 12:00:00
實施日期：1999/12/31 12:00:00
中國標準分類號：G04
國際標準分類號：71.040.50
引用標準：DIN 50014;DIN 55672-1-2007;DIN 55672-2;DIN EN ISO 1513;DIN EN ISO 15528;DIN ISO 5725-1;DIN ISO 3696
適用范圍：This standard describes the determination of the molecular weight distribution, number-average molecularweight Mn and weight-average molecular weight Mw of polymers that are soluble in water by gel permeationchromatography (GPC).This standard is applicable to neutral polymers and polyanions (e.g. polycarboxylates, polysaccharides,completely saponified polyvinyl alcohols and high-molecular polyethylene oxides). It is not applicable topolycations (e.g. polyvinytpyrrolidone, polyvinylpyridine, salts of poly(diallyI-N,N-dimethyl-azacyclopentane)and chitosane.It is possible that, in spite of the good solubility in the mobile phase and the good repeatability of thechromatograms obtained, this method cannot be used with certain polymer types because of specificinteractions, such as adsorption, within the sample/eluent/column combination (see also clause 12).The method is not an absolute one and requires calibration with commercially available unbranched pullulanstandards that have been characterized by independent absolute methods. The results for samples ofpolymers other than pullulan are therefore only comparable within groups of samples of the same type.The conditions specified in this standard are not suitable for the GPC analysis of polymer samples with Mwvalues greater than 10~6 g/mol (see Annex C).No correction methods (e.g. for the elimination of peak broadening) are included in this standard. If absolutemolecular weight values are required, an absolute method, e.g. membrane osmometry for Mn or lightscattering for Mw, shall be used.