標準號：BS EN 12821-2009
中文標準名稱：食品.高效液相色譜法測定維生素D含量.維生素D3或鈣化醇(維生素D2)的測定
英文標準名稱：Foodstuffs - Determination of vitamin D by high performance liquid chromatography - Measurement of cholecalciferol (D3) or ergocalciferol (D2)
標準類型：X04
發布日期：2009/4/30 12:00:00
實施日期：2009/4/30 12:00:00
中國標準分類號：X04
國際標準分類號：67.050
引用標準：EN ISO 3696
適用范圍：This European Standard specifies a method for the determination of vitamin D3 (cholecalciferol) or vitamin D2(ergocalciferol) in foodstuffs by high performance liquid chromatography (HPLC).Vitamin D3 is primary in foodstuffs of animal origin, while vitamin D2 is primary in wild mushrooms. Bothvitamin D3 and vitamin D2 can be present in fortified foodstuffs. This European Standard is not applicable forsamples with a content of vitamin D3 and vitamin D2.Apart from the vitamin D activity from the parent forms, vitamin D3 and vitamin D2, the correspondingmetabolites 25-hydroxy vitamin D and 1,25-dihydroxy vitamin D also contribute to the vitamin D activity. ThisEuropean Standard does only include measurement of vitamin D3 or vitamin D2.This European Standard provides the base for the analytical methods. It is intended to serve as a frame inwhich the analyst can define his own analytical work in accordance to the standard procedure.This method has been validated in inter-laboratory tests on fortified and non-fortified samples such asmargarine, milk, milk powder, liquid infant formula, infant formula, cooking oil, and fish oil at levels from 0,4μg/100 g to 14 μg/100 g. Further information on the validation data is given in Annex D.